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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human MAGEA3 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, FC (Intra), ELISA
- 靶点:
MAGEA3
- 抗体英文名:
MAGEA3 Recombinant monoclonal antibody
- 抗体名:
MAGEA3 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | A375 cells, Jurkat cells, SGC-7901 cells, NCI-H1299 cells |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83480-5-RR targets MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0265 Product name: Recombinant human MAGEA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC000340 Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | melanoma antigen family A, 3 |
| 别名 | HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D |
| 计算分子量 | 314 aa, 35 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC000340 |
| 基因名称 | MAGEA3 |
| Gene ID (NCBI) | 4102 |
| RRID | AB_3671108 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43357 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.
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文献和实验ml)lml,再从鸡的翼下静脉取血0.5--1ml,取出后放入刻度离心管中,再加入2--3ml Alsver液,使总量为4--5ml,混匀并封口,置于4℃冰箱内可供一周内使用,实验时取贮备的鸡血细胞lml,加入4ml85%生理盐水,混匀后以1200rr/min离心5min,去上清液后,最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次,最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞,可先用0.9%
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