- ￥3580
- Proteintech
- G14464-1-5C
- 2025年10月09日
- Single Cell (Intra)
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TCF7 protein
- 亚型：
IgG
- 保存条件：
2-8°C Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
5CFLX
- 适应物种：
Human
- 宿主：
Rabbit
- 应用范围：
Single Cell (Intra)
- 靶点：
TCF7
- 抗体英文名：
MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal)
- 抗体名：
MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal)
- 规格：
10ug
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G14464-1-5C targets TCF7 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag5792 Product name: Recombinant human TCF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-384 aa of BC048769 Sequence: MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLEDGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTGGKRNAFGTYPEKAAAPAPFLPMTVL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Polyclonal |
| 全称 | MultiPro® 5CFLX Anti-Human TCF7 (Polyclonal) |
| 别名 | T cell factor 1, TCF 1, TCF 7, TCF1, TCF7, Transcription factor 7 |
| 计算分子量 | 42 kDa |
| GenBank蛋白编号 | BC048769 |
| 基因名称 | TCF1/TCF7 |
| Gene ID (NCBI) | 6932 |
| ENSEMBL Gene ID | ENSG00000081059 |
| RRID | AB_3673888 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCGCCTATAGAGACGCCCCATATAAGAAA |
| 条形码序列 | CGCCTATAGAGACGC |
| 形式 | Liquid |
| UNIPROT ID | P36402 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
minutes at 60°C with mixing. Insoluble material is pelleted by centrifugation for 15 minutes at 5000xg. Add DABCO (Diazabicyclo(2.2.2.) octane. Merck® #803456) to final 2.5% to the solution as anti-bleaching agent
or overnight at 4°C with agitation. For a mouse monoclonal antibody, a goat anti-mouse HRP-conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat anti-rabbit HRP-conjugated antibody is advisable. Wash the membrane five times
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