- ￥3580
- Proteintech
- G20189-1-5C
- 2025年10月09日
- Single Cell (Intra)
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MAFB protein
- 亚型：
IgG
- 保存条件：
2-8°C Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
5CFLX
- 适应物种：
Human
- 宿主：
Rabbit
- 应用范围：
Single Cell (Intra)
- 靶点：
MAFB
- 抗体英文名：
MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)
- 抗体名：
MultiPro® 5CFLX Anti-Human MAFB (Polyclonal)
- 规格：
10ug
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G20189-1-5C targets MAFB in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag14102 Product name: Recombinant human MAFB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 168-323 aa of BC036689 Sequence: QASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Polyclonal |
| 全称 | MultiPro® 5CFLX Anti-Human MAFB (Polyclonal) |
| 别名 | KRML, Maf B, MAFB, Transcription factor MafB |
| 计算分子量 | 323 aa, 36 kDa |
| GenBank蛋白编号 | BC036689 |
| 基因名称 | MAFB |
| Gene ID (NCBI) | 9935 |
| ENSEMBL Gene ID | ENSG00000204103 |
| RRID | AB_3673893 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTCGCGGACCAGGAATCCCATATAAGAAA |
| 条形码序列 | TCGCGGACCAGGAAT |
| 形式 | Liquid |
| UNIPROT ID | Q9Y5Q3 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
minutes at 60°C with mixing. Insoluble material is pelleted by centrifugation for 15 minutes at 5000xg. Add DABCO (Diazabicyclo(2.2.2.) octane. Merck® #803456) to final 2.5% to the solution as anti-bleaching agent
or overnight at 4°C with agitation. For a mouse monoclonal antibody, a goat anti-mouse HRP-conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat anti-rabbit HRP-conjugated antibody is advisable. Wash the membrane five times
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