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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
recombinant protein
- 宿主:
Rabbit
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
HA Tag
- 抗体英文名:
CoraLite® Plus 488-conjugated HA Tag Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated HA Tag Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81290 targets HA Tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 文献引用应用 | IF |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | HA Tag |
| 别名 | HA |
| 基因名称 | HA Tag |
| Gene ID (NCBI) | 6 |
| RRID | AB_3086562 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | HATAG |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Int J Biol Macromol SIRT5 desuccinylating IDH2 to alleviate oxidative stress in bovine mammary epithelial cells induced by ammoniaAuthors - Shikai Gao |
-Glu-Glu-Asp-Leu,这11个氨基酸作为抗原表位表达在不同的蛋白质框架中仍可识别其相应抗体。C-Myc tag已成功应用在 Western-blot杂交技术、免疫沉淀和流式细胞计量术中, 可用于检测重组蛋白质在靶细胞中的表达。 eGFP eGFP标签蛋白,是增强型绿色荧光蛋白eGFP,激发波长为488nm,发射波长为507nm,其是由野生型绿色荧光蛋白GFP通过氨基酸突变和密码子优化而来的。相对于GFP,eGFP荧光强度更强、荧光性质更稳定。同时载体中构建
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
货号T501,产品描述:HA-Tag Rabbit Polyclonal Antibody;规格 50 ul / 100 ul; 货号T503,产品描述:Anti-DYKDDDDK Polyclonal Antibody;规格 50 ul / 100 ul; 货号T504,产品描述:Myc-Tag Mouse Monoclonal Antibody;规格 50 ul / 100 ul; 货号
1的配体可保护DD融合蛋白免受降解,使融合蛋白在细胞内累积。稳定作用只需15-30分钟即可完成,让用户可监控快速诱导蛋白的影响,比传统的四环素诱导方法更快。 多功能的标签 若您还未考虑好该用什么颜色,或者希望使用一些更灵活的标签,那么也有很多产品可以选择,比如Promega的HaloTag®、Life Technologies的Lumio,以及NEB的SNAP-tag、CLIP-tag、ACP-tag和 MCP-tag等。每个系统都是独特的,但基本原理一致,将目的
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