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CoraLite® Plus 647-conjugated

Ki-67 多克隆抗体 CL647-28074
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  • ¥3200
  • Proteintech已认证
  • CL647-28074
  • 2025年10月09日
  • IF/ICC, IF-P, FC (Intra)
  • Rabbit
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant mouse ki67 protein

    • 亚型

      IgG

    • 保存条件

      Store at -20°C. Avoid exposure to light. Stable for one year after shipment.

    • 克隆性

      Polyclonal

    • 标记物

      CoraLite® Plus 647 Fluorescent Dye

    • 适应物种

      mouse

    • 宿主

      Rabbit

    • 应用范围

      IF/ICC, IF-P, FC (Intra)

    • 靶点

      Ki-67

    • 抗体英文名

      CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody

    • 抗体名

      CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody

    • 规格

      100ul

    经过测试的应用

    Positive IF-P detected inmouse colon tissue
    Positive IF/ICC detected inHeLa cells
    Positive FC (Intra) detected inNIH/3T3 cells, Jurkat cells
    For other applications, we recommend the unconjugated version of this antibody, 28074-1-AP

    推荐稀释比

    应用推荐稀释比
    Immunofluorescence (IF)-PIF-P : 1:50-1:500
    Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
    Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    CL647-28074 targets Ki-67 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with mouse samples.

    经测试应用 IF/ICC, IF-P, FC (Intra)
    经测试反应性 mouse
    免疫原

    CatNo: Ag27894

    Product name: Recombinant mouse ki67 protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 2777-3005 aa of NM_001081117

    Sequence: MFQSSPRRPRTRRGKVEADEEPSAVRKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQISDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSARDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVST

    宿主/亚型 Rabbit / IgG
    抗体类别 Polyclonal
    产品类型 Antibody
    全称 antigen identified by monoclonal antibody Ki 67
    别名 Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen
    计算分子量 351 kDa
    GenBank蛋白编号NM_001081117
    基因名称 ki67
    Gene ID (NCBI) 17345
    RRIDAB_3673572
    偶联类型 CoraLite® Plus 647 Fluorescent Dye
    最大激发/发射波长654 nm / 674 nm
    形式Liquid
    纯化方式Antigen affinity purification
    UNIPROT IDE9PVX6
    储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
    储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

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    图标文献和实验
    相关实验
    • 流式细胞方案

      ')2 Fragment(Alexa Fluor® 555 Conjugate)#4413  Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠:  Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408  Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor

    • Western Blot 实验试剂选购指南

      647 34079 SuperSignalâ West Pico Chemiluminescent Substrate Trial Kit 50

    • R&D Systems支原体污染检测新方法(ELISA)专辑

      MycoProbe®试剂盒的详细介绍 1. 试剂盒成分:     细胞裂解液 ,Cell Lysis Buffer     杂交板96孔板, Hybridization Plate     链亲和素捕获板96孔板, Streptavidin Capture Plate     样品稀释液 ,Sample Diluents     碱性磷酸酶标记的抗地高辛检测抗体 ,Anti-digoxigenin conjugated to alkaline phosphatase    

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