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- 文献和实验
- 技术资料
- 免疫原:
Recombinant mouse ki67 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
CoraLite® Plus 647 Fluorescent Dye
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
IF/ICC, IF-P, FC (Intra)
- 靶点:
Ki-67
- 抗体英文名:
CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody
- 抗体名:
CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF-P detected in | mouse colon tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | NIH/3T3 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-28074 targets Ki-67 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag27894 Product name: Recombinant mouse ki67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2777-3005 aa of NM_001081117 Sequence: MFQSSPRRPRTRRGKVEADEEPSAVRKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQISDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSARDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVST |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | antigen identified by monoclonal antibody Ki 67 |
| 别名 | Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen |
| 计算分子量 | 351 kDa |
| GenBank蛋白编号 | NM_001081117 |
| 基因名称 | ki67 |
| Gene ID (NCBI) | 17345 |
| RRID | AB_3673572 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | E9PVX6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.
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文献和实验')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
(终末耗竭)的对比,并可加入CXCR6以定位肿瘤微环境中靠近树突状细胞的T细胞。胞内染色对检测转录因子(如TCF1、TOX、EOMES、T-BET等)及磷酸化信号蛋白(p-STAT5/3)至关重要。此外,流式还可同步评估增殖(Ki-67/CFSE)、细胞因子分泌(IFN-γ、TNF-α、IL-2减少)及线粒体功能(MitoTracker),全面刻画耗竭T细胞的功能缺陷。菲恩生物提供专业流式配色方案与高品质抗体,优化多色检测,提升灵敏度与准确性,助力免疫、肿瘤及自身免疫病研究,诚邀合作共促创新
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