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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Multi-Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, ELISA
- 靶点:
CD9
- 抗体英文名:
Multi-rAb® CD9 Multi-Recombinant antibody
- 抗体名:
Multi-rAb® CD9 Multi-Recombinant antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | A431 cells, L02 cells, human placenta tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00018 targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | CD9 molecule |
| 别名 | 5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen |
| 计算分子量 | 25 kDa |
| 观测分子量 | 23-30 kDa |
| GenBank蛋白编号 | NM_001769.4 |
| 基因名称 | CD9 |
| Gene ID (NCBI) | 928 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P21926 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).
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文献和实验.) 0.1M sodium cacodylate - 10.7g in 500mls of distilled water. 0.2M sodium cacodylate - 21.4g in 500mls of distilled water. 0.4M sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® )
电镜样品制备方法 Specimen preparation for electron microscopy
sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® ) (Supplier: Agar Scientific, 66A Cambridge Road, Stansted Essex, CM24 8DA, U.K.) Agar 100 resin - 24g. DDSA (E.M. grade) - 13g. MNA (E.M
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