- ￥1350 - 3000
- Proteintech
- 29906-1-AP
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TSC1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Hamartin/TSC1
- 抗体英文名：
Hamartin/TSC1 Polyclonal antibody
- 抗体名：
Hamartin/TSC1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, mouse kidney tissue, MCF-7 cells |
| Positive IHC detected in | mouse kidney tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29906-1-AP targets Hamartin/TSC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag31621 Product name: Recombinant human TSC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 965-1164 aa of NM_000368 Sequence: LYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tuberous sclerosis 1 |
| 别名 | TSC1, TSC 1, TSC, LAM, KIAA0243 |
| 计算分子量 | 130KD |
| 观测分子量 | 140-145 kDa |
| GenBank蛋白编号 | NM_000368 |
| 基因名称 | Hamartin/TSC1 |
| Gene ID (NCBI) | 7248 |
| RRID | AB_2918352 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC1, also named as hamartin, interacts with TSC2 to form a protein complex that inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed: 12271141, PubMed: 28215400). It can recruit TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed: 16464865, PubMed: 29127155). The loss of TSC1/TSC2 in mouse neurons resulted in a block in oligodendrocyte hypomyelination in vivo (PubMed: 28183733).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IHC | Genes Dis Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized miceAuthors - Liying Shan |
| human | WB | Elife A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progressionAuthors - Chaojun Yan |
| human | WB | Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progressionAuthors - Mengmeng Zhang |
| human | WB,IP,IHC,IF | Br J Cancer CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitorsAuthors - Xinlin Liu |
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