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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Sandwich ELISA, Indirect ELISA
- 靶点:
Complement factor H/CFH
- 抗体英文名:
Complement factor H/CFH Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Complement factor H/CFH Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
85132-3-PBS targets Complement factor H/CFH as part of a matched antibody pair:
MP01827-2: 85132-1-PBS capture and 85132-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3753 Product name: Recombinant Human Complement factor H protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 860-1231 aa of Sequence: SIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | complement factor H |
| 别名 | CFH, Complement factor H, ARMD4, ARMS1, Factor H |
| 计算分子量 | 139kDa |
| 基因名称 | Complement factor H |
| Gene ID (NCBI) | 3075 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08603-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验洗涤2次,离心弃上清,真空抽干。 (5)加入适量1×TE溶解。如此可得线性化载体 DNA 。 (6)测定 DNA 的含量。 (7)加入线性载体 DNA 和含量3~4倍于载体的待插入 DNA 片段,连接缓冲液及T4连接酶适量至总体积为20μl,在12~14℃反应12~16h。 (8)连接反应液可置-20℃保存,供转化用。 (9)关于待插入 DNA 片段的获得参见附注。 (二)大肠杆菌感受态的制备及重组 DNA 的转化
Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
., Lewis, K.A., Perrin, M.H., and Vale, W.W. 1993. Expression cloning of a human corticotropin‐releasing‐factor receptor. Proc. Natl. Acad. Sci. U.S.A. 90:8967‐8971. De Souza, E
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
van den Elsen, J.M.H., Martin, A., Wong, V., Clemenza, L., Rose, D.R., and Isenman, D.E. 2002. X‐ray crystal structure of the C4d fragment of human complement component C4. J. Mol. Biol. 322:1103‐1115
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