- ￥1350 - 3000
- Proteintech
- 28270-1-AP
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CALM2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Calmodulin 1/2/3
- 抗体英文名：
Calmodulin 1/2/3 Polyclonal antibody
- 抗体名：
Calmodulin 1/2/3 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28270-1-AP targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag28000 Product name: Recombinant human CALM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 72-149 aa of BC006464 Sequence: MMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calmodulin 2 (phosphorylase kinase, delta) |
| 别名 | CALM2, CALM, CALM1, CALM3, CALML2 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17-22 kDa |
| GenBank蛋白编号 | BC006464 |
| 基因名称 | Calmodulin 1 |
| Gene ID (NCBI) | 805 |
| RRID | AB_3669650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62158 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).
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文献和实验Purification of Calmodulin from Bovine Brain
References: Watterson et al.(1980)J Biol Chem 255:962-75. Charbonneau et al.(1979)Biochem Biophys Res Commun 90:1039-47. Extraction of Calmodulin Solutions: 1.1 N NH4OH stored at room temperature. 2.1 N sulfuric acid in 50% AmSO4 stored at room
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
600, then dilute the cells to final OD600 of 0.5, place on ice. Screening Plaques with AP-Fusion Protein Reagents: 1M IPTG stock solution NZY plates with phage 20 x
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