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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG3
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
megathura crenulata
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
Mouse IgG3 isotype control
- 抗体英文名:
Mouse IgG3 isotype control Monoclonal antibody
- 抗体名:
Mouse IgG3 isotype control Monoclonal antibody
- 规格:
100ul/500ul/1ml
| 规格: | 100ul | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥4000.0 |
| 规格: | 1ml | 产品价格: | ¥6000.0 |
经过测试的应用
| Positive WB detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66360-4-Ig targets Mouse IgG3 isotype control in WB, ELISA applications and shows reactivity with megathura crenulata samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | megathura crenulata |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Mouse IgG3 isotype control |
| 别名 | Mouse IgG3 control, IgG3 isotype, mouse IgG control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2923630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody is used as the isotype control of mouse IgG3. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc
The history of isotype selection for therapeutic antibodies clearly demonstrates that solid understanding of the biologic effects mediated by immunoglobulin (Ig) subclasses IgG1, IgG2, IgG3, and IgG4 and their variants is a prerequisite
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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