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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
Staining Procedure for Flow Cytometric Detection of Human Cyclins
unconjugated isotype controls and mAbs, add 20 m l of FITC-conjugated goat anti-mouse Ig (Cat. No. 12064D) at optimal working dilution. Incubate 30 min at RT in the dark. Add 2 ml Wash buffer, centrifuge 5 min at 200 x g Aspirate supernatant. 10
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