- ￥3600 - 5000
- Proteintech
- 98616-1-PBS
- 2025年10月09日
- FC
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CD15
- 抗体英文名：
Anti-Human CD15 Rabbit Recombinant Antibody, PBS Only
- 抗体名：
Anti-Human CD15 Rabbit Recombinant Antibody, PBS Only
- 规格：
500ug/1mg
| 规格： | 500ug | 产品价格： | ￥3600.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥5000.0 |
产品信息
98616-1-PBS targets CD15 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD15 |
| 别名 | Lewis X, SSEA-1 |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD15, also known as stage-specific embryonic antigen-1 (SSEA-1) or Lewis X (LeX), is a cell surface glycan consisting of a trisaccharide with the structure Galβ1-4[Fucα1-3]GlcNAc (PMID: 18953356). CD15 is expressed on most terminally differentiated myeloid cells, including neutrophils, eosinophils, monocytes, macrophages, mast cells, and Langerhans cells. It has also been shown to be expressed on stem cells and progenitors in the embryonic and adult nervous system, Hodgkin's lymphoma cells, and cells from a variety of solid cancers (PMID: 19185848). CD15 acts as a ligand for selectins in transendothelial migration and is involved in phagocytosis and chemotaxis (PMID: 1362195).
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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