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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human ADC protein
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite®594 Fluorescent Dye
- 适应物种:
Human, mouse, pig, rat
- 宿主:
Mouse
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
ADC
- 抗体英文名:
CoraLite®594-conjugated ADC Monoclonal antibody
- 抗体名:
CoraLite®594-conjugated ADC Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67884 targets ADC in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, pig, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | Human, mouse, pig, rat |
| 免疫原 |
CatNo: Ag31049 Product name: Recombinant human ADC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 251-460 aa of BC028128 Sequence: EIASVINSALDLYFPEGCGVDIFAELGRYYVTSAFTVAVSIIAKKEVLLDQPGREEENGSTSKTIVYHLDEGVYGIFNSVLFDNICPTPILQKKPSTEQPLYSSSLWGPAVDGCDCVAEGLWLPQLHVGDWLVFDNMGAYTVGMGSPFWGTQACHITYAMSRVAWEALRRQLMAAEQEDDVEGVCKPLSCGWEITDTLCVGPVFTPASIM |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | arginine decarboxylase |
| 别名 | AZIN2, AzI2, Arginine decarboxylase, ARGDC, Antizyme inhibitor 2 |
| 计算分子量 | 460 aa, 50 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC028128 |
| 基因名称 | ADC |
| Gene ID (NCBI) | 113451 |
| RRID | AB_2934784 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96A70 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.
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文献和实验明显膨大,以手触摸时,皮肤有紧张感,即可用16号针头采集腹水,一般可连续采2-3次,通常每只小鼠可采5-10ml腹水;d. 将腹水离心(2000r/min 5分钟),除去细胞成分和其他的沉淀物,收集上清,测定抗体效价,分装,-70℃冻存备用,或冻干保存。二、体外培养生产单抗的方法总体上讲,杂交瘤细胞系并不是严格的贴壁依赖细胞(anchorage dependent cell,ADC),因此既可以进行单层细胞培养,又可以进行悬浮培养。杂交瘤细胞的单层细胞培养法是各个实验室最常用的手段,即将杂交
Nature 综述导读:FDA 批准第 100 个抗体药物!抗体药物的研究方向在哪里?
图一从 1986 年 FDA 批准第一批单克隆抗体起已有 35 年的历史,抗体类药物每年占 FDA 新药批准的近五分之一。在这段时间里,抗体工程发生了巨大的变化。目前的抗体药物因具有高特异性,低不良反应的特点已成为近年来开发的主要一类新药。在过去的 5 年里,抗体已经成为医药市场上最畅销的药物。预估 2021 年全球十大畅销的药物中,就会有 5 个抗体类药物(如图二)。因此,随着抗体类药物被批准用于治疗各种包括癌症、自身免疫、代谢和传染病,治疗性抗体药物的市场必会呈现爆炸式增长的态势。 图二
Anti-Digoxin 产品详情 Dylight 594标记小鼠抗地高辛Digoxin单克隆抗体 Dylight 594-conjugated IgG Fraction Monoclonal Mouse Anti-Digoxin 产品详情
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