- ￥1500 - 3280
- Proteintech
- 80698-1-RR
- 2025年10月09日
- WB, ELISA
- Rabbit
- Human
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- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
Human
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
Phospho-TSC2 (Thr1462)
- 抗体英文名：
Phospho-TSC2 (Thr1462) Recombinant monoclonal antibody
- 抗体名：
Phospho-TSC2 (Thr1462) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80698-1-RR targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tuberous sclerosis 2 |
| 别名 | FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein |
| 计算分子量 | 1807 aa, 201 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC150300 |
| 基因名称 | TSC2 |
| Gene ID (NCBI) | 7249 |
| RRID | AB_3085354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49815 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
defined by their ability to form plaques on E. coli P2 lysogens.Commonly used bacterial strainsC600- F-, e14, mcrA, thr-1 supE44, thi-1, leuB6, lacY1, tonA21, l--for plating lambda (gt10) libraries, grows well in L broth, 2x TY, plate on NZYDT+Mg.-Huynh
- Refers to red-gam- mutant derivatives of lambda defined by their ability to form plaques on E. coli P2 lysogens. Commonly used bacterial strains C600 - F-, e14, mcrA, thr-1 supE44, thi-1, leuB6, lacY1, tonA21, l- -for plating lambda (gt10) libraries
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