- ￥940 - 2350
- Proteintech
- FITC-65277
- 2025年10月09日
- FC (Intra)
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
FITC Plus Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC (Intra)
- 靶点：
IL-12A/IL-12 p35
- 抗体英文名：
FITC Plus Anti-Human IL-12A/IL-12 p35 (1E12H6)
- 抗体名：
FITC Plus Anti-Human IL-12A/IL-12 p35 (1E12H6)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥940.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2350.0 |
经过测试的应用
| Positive FC (Intra) detected in | LPS and Brefeldin A treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65277 targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human |
| 免疫原 | fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| 别名 | IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35 |
| 计算分子量 | 25 kDa |
| GenBank蛋白编号 | BC104982 |
| 基因名称 | IL-12A |
| Gene ID (NCBI) | 3592 |
| RRID | AB_3085007 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P29459 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).
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文献和实验Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
Immunohistochemistry: Fluorescence Protocol 2.0
temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 , IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5% human AB serum. Cells are ready for analysis by flow cytometry
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
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