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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IL-4R protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
IL-4RA/CD124
- 抗体英文名:
IL-4RA/CD124 Monoclonal antibody, PBS Only (Detector)
- 抗体名:
IL-4RA/CD124 Monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
67051-5-PBS targets IL-4RA/CD124 as part of a matched antibody pair:
MP50242-3: 67051-3-PBS capture and 67051-5-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28411 Product name: Recombinant human IL-4R protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-232 aa of NM_000418 Sequence: MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 4 receptor |
| 别名 | IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein |
| 计算分子量 | 90 kDa |
| GenBank蛋白编号 | NM_000418 |
| 基因名称 | IL-4R |
| Gene ID (NCBI) | 3566 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P24394 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.
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文献和实验a. 制备活性较好的细胞悬液,调节浓度至107个/ml。 b. 在小试管(5×50mm)中加入100ul细胞悬液,再加入100ul待检McAb的样品,混匀,4℃作用30-90分钟。 c. 用洗涤液(PBS 900ml,小牛血清50ml,4%NaN3 50ml)洗二次,每次加洗涤液1-5ml,1000r/min离心5分钟,弃上清。加入100ul荧光抗体,4℃作用30-90分钟。 d. 同法洗涤,将细胞重新悬于20-30ul含10%甘油和10-100ug苯二胺/ml
4 LPL)显著多于CD 8 阳性细胞(CD 8 LPL)。40%CD 4 LPL表达CD 45 RO抗原,能产生大量IL-2、IL-4和γ-INF,为记忆性T淋巴细胞,能辅助B细胞转化成抗体产生细胞并促其分泌抗体。60%CD 4 LPL表达CD 45 RA和CD 8 抗原,能直接抑制抗体产生细胞转化和分泌。这两种细胞的比例在调节 抗体分泌中
; 2、一抗用多克隆抗体易出现非特异性着色,建议用单克隆抗体看看; 3、内源性过氧化物酶和生物素在肝脏、肾脏等组织含量很高(含血细胞多的组织),需要通过延长灭活时间和增加灭活剂浓度来降低背景染色; 4、非特异性组分与抗体结合,这需要通过延长二抗来源的动物免疫血清封闭时间和适当增加浓度来加强封闭效果; 5、缩短 DAB 孵育时间或降低 DAB 浓度/过氧化氢浓度等; 6、适当增加 PBS 冲洗次数和浸洗时间,在一抗、二抗或 SP 孵育之后的浸洗尤为重要; 7、防止标本染色过程中
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