- ￥5800
- Proteintech
- 86067-4-PBS
- 2025年10月09日
- WB, Indirect ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, Indirect ELISA
- 靶点：
Serpin C1/Antithrombin III
- 抗体英文名：
Serpin C1/Antithrombin III Recombinant monoclonal antibody, PBS Only
- 抗体名：
Serpin C1/Antithrombin III Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
86067-4-PBS targets Serpin C1/Antithrombin III in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Eg3100 Product name: recombinant human SERPINC1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 33-464 aa of NM_000488.4 Sequence: HGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade C (antithrombin), member 1 |
| 别名 | Antithrombin III, Serpin C1, SERPINC1, Antithrombin-III, AT3 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | NM_000488.4 |
| 基因名称 | Serpin C1/Antithrombin III |
| Gene ID (NCBI) | 462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01008 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
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文献和实验A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS : 1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide) 2. 7-Amino-actinomycin D (7-AAD, e.g., Calbiochem, CA) 3. Human AB serum, heat-inactivated
至 1 升。室温保存。 2. 甲醛溶液（无甲醇的）。 3. 孵育缓冲液：将 0.5 g 牛血清白蛋白（bovine serum albumin，BSA）溶解在 100 ml 的 1 X PBS 中。保存在 4°C。 B. 固定 1. 离心收集细胞，弃上清。 2. 将细胞重悬在 0.5-1 ml PBS 中。加入甲醛溶液至终浓度为 2-4%。 3. 37°C 固定 10 分钟。 4. 放在冰上降温 1 分钟。 5. 做细胞外染色不需要细胞膜打孔时，继续 D1
Preparation and Staining of Paraffin Sections
the jar tightly, and allow the solution to slowly cool to room temperature for 20 minutes. Note: It is important to let the temperature ramp down slowly to allow the protein molecules to fold properly. Rinse slides in PBS 3X, 5 minutes each time
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