- ￥5800
- Proteintech
- 67182-1-PBS
- 2025年10月09日
- WB, Indirect ELISA
- Mouse
- Human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PRKAG1 protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, Indirect ELISA
- 靶点：
AMPK Gamma 1
- 抗体英文名：
AMPK Gamma 1 Monoclonal antibody, PBS Only
- 抗体名：
AMPK Gamma 1 Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
67182-1-PBS targets AMPK Gamma 1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag28907 Product name: Recombinant human PRKAG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 165-321 aa of BC000358 Sequence: LYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, AMP-activated, gamma 1 non-catalytic subunit |
| 别名 | AMPK Gamma 1, AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 36-38 kDa |
| GenBank蛋白编号 | BC000358 |
| 基因名称 | AMPK Gamma 1 |
| Gene ID (NCBI) | 5571 |
| RRID | AB_2882478 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P54619 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.
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文献和实验(4)称取Sephadex G25干粉0.3g，加入一支下口垫玻璃棉的5ml注射器外筒内;随后将上述交联物移入注射器外套;盖紧，室温作用(避光)3小时或4℃过夜。 (5)用少许PBS 将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟;再加入3/20V NaBH4溶液，混匀，室温作用1小时(或4℃过夜)。 (6)将交联物过Sephadex G200或Sepharose 6B(2.6×50cm)层析纯化，分管收集第一
)除去游离125I 用4％BSA PBS洗脱 ↓ 分部收集，0.5ml／管 每管取5μl进行γ计数，计算标记抗体的比活性和标记率 结合于McAb１２５I放射性强度(cpm) 标记率(％)＝─────────────────── 总放射性强度(cpm)
相关专题 生物帮之抗体制备 从小鼠腹水中纯化McAb的方法有盐析、离子交换层析 、凝胶过滤和亲和层析等。应用TSK DEAE-SPW离子交换层析柱从小鼠腹水中纯化McAb不仅纯化速度快，回收率和得率高，而且保持良好的生物学活性。 (一) 原理 根据离子交换原理，将经硫酸铵粗提的含McAbγ球蛋白上样结合于层析柱 上，通过增加洗脱液中的离子强度，洗脱并收集蛋白峰。 (二)操作
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