- ￥5800
- Proteintech
- 86207-1-PBS
- 2025年10月09日
- WB, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
DKK1
- 抗体英文名：
Mouse DKK1 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse DKK1 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
86207-1-PBS targets DKK1 as part of a matched antibody pair:
MP02491-1: 86207-1-PBS capture and 86207-2-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg4649 Product name: Recombinant Mouse DKK1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 32-272 aa of NM_010051.3 Sequence: TLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dickkopf homolog 1 (Xenopus laevis) |
| 别名 | Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, hDkk 1 |
| 计算分子量 | 29kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | NM_010051.3 |
| 基因名称 | Dkk1 |
| Gene ID (NCBI) | 13380 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O54908 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.
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文献和实验Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
较重要的是pH值和离子强度。PeproTech的细胞因子或重组蛋白在出厂前均经严格测试，说明上所标明的溶解液是能够将该细胞因子或重组蛋白完全溶解的液体。如果您所用的溶解液的pH值和离子强度与说明书中所标明的不符，很多时候会造成细胞因子或重组蛋白不能完全溶解或者根本无法溶解，这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述，而是根据习惯，直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗？ 答：有时可以，要看具体
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