- ￥1080 - 2160
- Proteintech
- APC-FcA98113
- 2025年10月09日
- FC
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
APC Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CD30 Ligand/TNFSF8
- 抗体英文名：
FcZero-rAb™ APC Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody
- 抗体名：
FcZero-rAb™ APC Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2160.0 |
经过测试的应用
| Positive FC detected in | Anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-FcA98113 targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0667 Product name: Recombinant Mouse CD30 Ligand/TNFSF8 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: N-6*His Domain: 68-239 aa of NM_009403.3 Sequence: QKKDSTPNTTEKAPLKGGNCSEDLFCTLKSTPSKKSWAYLQVSKHLNNTKLSWNEDGTIHGLIYQDGNLIVQFPGLYFIVCQLQFLVQCSNHSVDLTLQLLINSKIKKQTLVTVCESGVQSKNIYQNLSQFLLHYLQVNSTISVRVDNFQYVDTNTFPLDNVLSVFLYSSSD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 8 |
| 别名 | Tnfsf8, CD30 ligand, CD30-L, Tumor necrosis factor ligand superfamily member 8 |
| 计算分子量 | 27 kDa |
| GenBank蛋白编号 | NM_009403.3 |
| 基因名称 | Tnfsf8 |
| Gene ID (NCBI) | 21949 |
| 偶联类型 | APC Fluorescent Dye |
| 最大激发/发射波长 | 650 nm / 660 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32972 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
Characterization and Validation of Cre‐Driver Mouse Lines
(1:8000 dilution, VWR, cat. no. 69050‐3) was used with goat anti‐rabbit antibody coupled to horseradish peroxidase (1:100 dilution; Invitrogen, cat. no. G‐21234) as secondary antibody. After washing, Cre was visualized by FITC‐tyramide amplification (1/50
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