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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
- 靶点:
Acetyl-Histone H2B (Lys120)
- 抗体英文名:
Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody
- 抗体名:
Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | Trichostatin A treated NIH/3T3 cells |
| Positive IF/ICC detected in | Trichostatin A treated HeLa cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 2, H2be |
| 别名 | Acetyl Histone, H2B Ac Lys120, H2B Acetyl K120, H2B Acetyl Lys120, H2B Acetyl Lysine120 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC005827 |
| 基因名称 | Histone H2B |
| Gene ID (NCBI) | 8349 |
| RRID | AB_3672059 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q16778 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).
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文献和实验组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白;在细胞正常 pH 值时,组蛋白带有正电荷,这样它们就可以和 DNA 结合,这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实,组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg , Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白: H1 , H2A , H2B , H3
IHC IC2575Acetyl-Histone H2B (Lys12) AntibodyRabbitW IHC IC2571Acetyl-Histone H2B (Lys20) AntibodyRabbitW IP IHC IC2574Acetyl-Histone H2B (Lys5) AntibodyRabbitW IP IHC IC9675Acetyl-Histone H3 (Lys18) AntibodyRabbitW IHC IC9674Acetyl-Histone H3 (Lys23
通讯作者。此项研究由科技部863计划和北京市科委资助,在北京生命科学研究所完成。 论文"Partition of histone H3/H4 tetramers during DNA replication dependent chromatin assembly "引起关注,science配发了Geneviève Almouzni博士题名为"Mixing or not mixing”的文章,对此项工作进行了评论与展望。评论指出“尽管早期研究认为,组蛋白H2A-H2B可以在核小体之间交换而H3-H4四
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