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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, ELISA
- 靶点:
AFP
- 抗体英文名:
AFP Recombinant monoclonal antibody
- 抗体名:
AFP Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells, human placenta |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82982-5-RR targets AFP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | alpha-fetoprotein |
| 别名 | Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin |
| 计算分子量 | 69 kDa |
| 观测分子量 | 68-72 kDa |
| GenBank蛋白编号 | BC027881 |
| 基因名称 | AFP |
| Gene ID (NCBI) | 174 |
| RRID | AB_3670731 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P02771 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)
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文献和实验1原理 本试剂盒采用针对不同抗原决定簇的双单克隆抗体分别制备成包被板和酶结合物,和用ELISA双抗体夹心法原理测人血清中AFP含量。适用于原发性肝细胞癌及胎儿神经管发育异常(脊柱裂、脑积水)的早期辅助诊断。 2标本采集: 21标本采集前病人准备:受者应空腹 22标本种类:血清或血浆 23标本要求: 病人标本无需特殊制备处理 3标本储存于运输 31标本的储存:待测样本室温不超过8小时,4-8℃不超过72小时,-20℃可长期保存,避免反复
,通过硫酸铵沉淀,获得纯度较高的单抗,通过SDS-PAGE检测,由两个亚基组成,分子量分别为26 kD 和50 kD。 2.3 单克隆抗体活性的鉴定:抗原APT经过SDS-PAGE后,进行转移电泳,用丽春红S染色硝酸纤维素膜,证实抗原已转移到膜上并呈现出单一蛋白带,与3种单抗反应后,膜上出现棕色的与抗原APT特异结合的单克隆抗体带,与正常凝血酶原及其他血浆蛋白无交叉反应。 3 讨论 APT作为一种新的肝癌标志物对肝癌的早期诊断及预后具有重要的意义,对甲胎蛋白(AFP
》(PNAS)。该论文在线之际,受到国外Scientist,Nature news,The Australian,Thomson Reuters, Fox News, Agence France Presse (AFP法新社) 等美国、英国、俄罗斯、德国、巴西、印度各专业杂志及媒体的广泛关注和报道。 该研究表明由转基因水稻种子生产的重组人血清白蛋白(OsrHSA)在生理生化性质、物理结构,生物学功能、免疫原性与血浆来源的人血清白蛋白一致;并建 立了大规模生产重组人血清白蛋白的生产工艺,获得了高纯度和高
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