- ￥2800
- Proteintech
- CL555-60135
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- human, mouse, rat, pig
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human CHGA protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®555 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Chromogranin A
- 抗体英文名：
CoraLite®555-conjugated Chromogranin A Monoclonal antibody
- 抗体名：
CoraLite®555-conjugated Chromogranin A Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | PC-12 cells, SH-SY5Y cells, Neuro-2a cells |
| Positive FC (Intra) detected in | SH-SY5Y cells, Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-60135 targets Chromogranin A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag0807 Product name: Recombinant human CHGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 158-457 aa of BC006459 Sequence: MQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chromogranin A (parathyroid secretory protein 1) |
| 别名 | CHGA, CGA, EA-92, Chromogranin-A, AL-11 |
| 计算分子量 | 51 kDa |
| GenBank蛋白编号 | BC006459 |
| 基因名称 | Chromogranin A |
| Gene ID (NCBI) | 1113 |
| RRID | AB_2934644 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P10645 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
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