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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, Indirect ELISA
- 靶点:
CXCL3/GRO Gamma
- 抗体英文名:
CXCL3/GRO Gamma Recombinant monoclonal antibody, PBS Only
- 抗体名:
CXCL3/GRO Gamma Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
86261-1-PBS targets CXCL3/GRO Gamma in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Eg2982 Product name: recombinant human CXCL3 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 35-107 aa of NM_002090.3 Sequence: ASVVTELRCQCLQTLQGIHLKNIQSVNVRSPGPHCAQTEVIATLKNGKKACLNPASPMVQKIIEKILNKGSTN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) ligand 3 |
| 别名 | CXCL/GRO Gamma, CXCL3, C X C motif chemokine 3, C-X-C motif chemokine 3, GRO gamma |
| 计算分子量 | 11 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | NM_002090.3 |
| 基因名称 | CXCL3 |
| Gene ID (NCBI) | 2921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P19876 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CXC chemokine ligand 3 (CXCL3) is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression.
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文献和实验Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
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