- ￥5800
- Proteintech
- 66945-1-PBS
- 2025年10月09日
- WB, Indirect ELISA
- Mouse
- staphylococcus aureus
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
staphylococcus aureus
- 宿主：
Mouse
- 应用范围：
WB, Indirect ELISA
- 靶点：
Protein A
- 抗体英文名：
Protein A Monoclonal antibody, PBS Only
- 抗体名：
Protein A Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
66945-1-PBS targets Protein A in WB, Indirect ELISA applications and shows reactivity with staphylococcus aureus samples.
| 经测试应用 | WB, Indirect ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | staphylococcus aureus |
| 文献引用反应性 | yeast |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Protein A |
| 别名 | |
| GenBank蛋白编号 | EU695225 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2882269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.
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文献和实验| Species | Application | Title |
|---|---|---|
| yeast | WB | Proc Natl Acad Sci U S A Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stressAuthors - Siyu Fan |
少可以参考文献上给定的硫酸铵固体的溶解度表，《Guide to Protein Purification》一书中就有详细的溶解度表，静置两个小时左右，10000 g离心半个小时后，弃去上清，用PBS重新溶解沉淀，然后再次加入硫酸铵固体至45%饱和，静置两个小时左右10000 g离心半个小时，弃去上清，用少量PBS溶解沉淀，即得到较纯的抗体，因为此抗体中尚有较高浓度的硫酸铵，因此需要对PBS透析，一般透析一天一夜较合适，透析过程中更换透析液两至三次。如果对纯度要求不是很高，也可以不做第一次沉淀，直接用45
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
过夜。（5）用少许PBS将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟；再加入3/20V NaBH4溶液，混匀，室温作用1小时（或4℃过夜）。（6）将交联物过Sephadex G200或Sepharose 6B（2.6×50cm）层析纯化，分管收集第一峰。（7）酶结合物质量鉴定：克分子比值测定酶量（mg/ml）=OD403×0.4IgG量（mg/ml）=（OD280-OD403×0.3）×0.62克分子比值（E/P）=酶量×4/IgG量
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