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- Proteintech
- CL488-83929-2
- 2026年05月29日
- IF/ICC
- Rabbit
- human
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human 0741 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
IF/ICC
- 靶点：
Annexin V
- 抗体英文名：
CoraLite® Plus 488-conjugated Annexin V Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Annexin V Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83929-2 targets Annexin V in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0741 Product name: Recombinant human 0741 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-320 aa of BC001429 Sequence: MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | annexin A5 |
| 别名 | Annexin A5, ANXA5, 240621B10, Anchorin CII, Annexin 5 |
| 计算分子量 | 36 kDa |
| GenBank蛋白编号 | BC001429 |
| 基因名称 | Annexin V |
| Gene ID (NCBI) | 308 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08758 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
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文献和实验Apoptosis Kit 20T, 50T, 100T K071 Annexin V-FITC/7-AAD Apoptosis Kit 20T, 50T, 100T K072 Annexin V-APC/7-AAD Apoptosis Kit 20T, 50T, 100T K073 Annexin V-PE/7-AAD Apoptosis Kit 20T, 50T, 100T K074 Annexin V-FineTest®488/PI Apoptosis Kit 20T, 50T, 100
MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
from DiOC1(5). The example below is a protocol for labeling with an annexin V–Alexa Fluor® 488 conjugate and propidium iodide. 1) After step 3 (above), wash cells once by adding 2 mL of warm phosphate-buffered saline or other buffer to each tube
-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. 主要试剂 Alexa Fluor® 488 annexin V Propidium iodide PBS [Details
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