- ￥5800
- Proteintech
- 84682-1-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
Semaphorin 7A
- 抗体英文名：
Semaphorin 7A Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Semaphorin 7A Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
84682-1-PBS targets Semaphorin 7A as part of a matched antibody pair:
MP01498-1: 84682-2-PBS capture and 84682-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg2005 Product name: Recombinant Human SEMA7A protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 45-648 aa of NM_003612.5 Sequence: QGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| 别名 | SEMA7A, CD108, CDw108, John-Milton-Hargen human blood group Ag, Sema K1 |
| 计算分子量 | 75kDa |
| GenBank蛋白编号 | NM_003612.5 |
| 基因名称 | SEMA7A |
| Gene ID (NCBI) | 8482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75326-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验50μL。 封闭液：用PBS将试剂盒中的封闭存储液（Blocking stock solution R）稀释10倍，每孔200μL。 抗体稀释液：注意，只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液（Dilution buffer R）稀释10倍 检测抗体（Biotinylated detector antibody，Secondary antibody）：照U-Cytech说明书，加入双蒸水溶解。使用时，用抗体稀释液稀释100倍，每孔100μL。 酶联亲和
以非那西丁为探针采用高效液相色谱法快速测定细胞色素P450 1A2的酶活性
acid). The flow rate was 2.0 ml・min-1, and the UV detector was set at 244 nm. Firstly, phenacetin was incubated with human CYP 450 1A2 enzyme in vitro at 37℃ and stopped by addition of 50 μl acetonitrile and centrifuged (10 000 g) for 3 min. Finally
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