- ￥5800
- Proteintech
- 82989-1-PBS
- 2025年10月09日
- Sandwich ELISA, Indirect ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant mouse Hif1a protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
Sandwich ELISA, Indirect ELISA
- 靶点：
Hif-1 alpha
- 抗体英文名：
Mouse Hif-1 alpha Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse Hif-1 alpha Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
82989-1-PBS targets Hif-1 alpha as part of a matched antibody pair:
MP00056-2: 82989-1-PBS capture and 82989-3-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag33620 Product name: Recombinant mouse Hif1a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 530-825 aa of NM_001313919 Sequence: SEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQG* |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | hypoxia inducible factor 1, alpha subunit |
| 别名 | Hif1a, ARNT-interacting protein, Hif-1a, HIF1alpha, HIF-1-alpha |
| 计算分子量 | 93 kDa |
| GenBank蛋白编号 | NM_001313919 |
| 基因名称 | Hif1a |
| Gene ID (NCBI) | 15251 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q61221 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
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