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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human P2RX6 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
P2RX6
- 抗体英文名:
P2RX6 Recombinant monoclonal antibody
- 抗体名:
P2RX6 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84458-4-RR targets P2RX6 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12331 Product name: Recombinant human P2RX6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC033488 Sequence: MGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLEPQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWVDEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFNFSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSGCWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2X, ligand-gated ion channel, 6 |
| 别名 | P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor |
| 计算分子量 | 431 aa, 48 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC033488 |
| 基因名称 | P2RX6 |
| Gene ID (NCBI) | 9127 |
| RRID | AB_3671983 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O15547 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)
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文献和实验想。在所有细菌表达试验中,重组的成熟蛋白、多肽与cDNA编码无关的前酶或酶原前体均分别含有一个额外的108和80N末端残基。 2.酵母表达 首先构建pYELCS载体结合酿酒酵母中的铜离子敏感金属-硫蛋白基因启动子的表达系统。该系统可以表达少量的Der p2融合蛋白,得量为0.5mg/L,重组Der p2可被单克隆抗体柱层析。因得量少,并且表达的融合蛋白含有较多的非溶性Derp2,限制了与IgE的结合率。完整的Derpl在这个系统中却有很高的得量(40mg/1),均
抗体:Chapman MD与Amsterdam大学联合研制出用鼠抗Der p2单克隆抗体(Fab’)段与人源化重组IgE、IgGl和IgG4Fc段结合的嵌合抗体,后者既可以作为人类特异性变应原的探针,又能用于抗体反应的定性、定量测定。如抗Der p2嵌合IgE可用于IgE定量试验,通过绘制与IgE反应及抗Derp21gE反应的标准曲线,可计算出IgE的含量(ELISA)。该项实验适用于流行病学及免疫遗传学等领域研究,以及IgE对特异性变应原的反应及诊断学,变应原特异性嵌合抗体检测同型抗体的生物学功能也视为有价值
defined by their ability to form plaques on E. coli P2 lysogens.Commonly used bacterial strainsC600- F-, e14, mcrA, thr-1 supE44, thi-1, leuB6, lacY1, tonA21, l--for plating lambda (gt10) libraries, grows well in L broth, 2x TY, plate on NZYDT+Mg.-Huynh
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