- ￥1500 - 3280
- Proteintech
- 86238-2-RR
- 2026年05月29日
- WB, IHC, IF-P, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, ELISA
- 靶点：
Pan-Keratin (Type I)
- 抗体英文名：
Pan-Keratin (Type I) Recombinant monoclonal antibody
- 抗体名：
Pan-Keratin (Type I) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HaCaT cells, A431 cells, HT-29 cells, mouse skin tissue, rat skin tissue, rat colon tissue |
| Positive IHC detected in | human prostate cancer tissue, Human Kidney(renal cell) Cancer, human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cervical cancer tissue, human colon cancer tissue, human lung cancer tissue, mouse skin tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86238-2-RR targets Pan-Keratin (Type I) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Pan-Keratin (Type I) |
| 别名 | pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin, Type I Keratin (Acidic) |
| 观测分子量 | 45-65 kDa |
| 基因名称 | |
| Gene ID (NCBI) | Pan-Keratin (Type I) |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15323 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.
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文献和实验Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics
All eukaryotic cells contain three distinct cytoskeletal networks: microtubules, microfilaments, and intermediate filaments (IF). In epithelial cells in vivo, the IF cytoskeleton is composed of heteropolymers of type I (acidic) and type II
of yet unidentified modifier genes on the phenotype after the ablation of the respective keratin. To date, nearly all available keratin mouse models were generated by taking advantage of conventional gene-targeting strategies. To reveal their cell type
自杀性质粒载体：一般用于基因突变。将突变的目的基因克隆到自杀性质粒载体上，通过接合等使其进入宿主，由于在宿主菌中不存在复制基因启始所需的复制蛋白（如Pi蛋白等），其无法复制，在外界选择性压力的作用下,自杀性质粒载体所携带的突变基因就与宿主染色体上的野生型发生基因发生二次同源重组，产生了带有突变的突变株；而质粒载体自身由于自杀性特性连同染色体上原有的野生型基因一起随着细菌的传代从菌体内消失。至于一次重组的突变株，可借助质粒上的某些选择基因（如sacB，sucrose敏感）筛除。整合载体：将目的
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