- ￥5800
- Proteintech
- 86073-5-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA, Sample test
- Rabbit
- rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
rat
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Indirect ELISA, Sample test
- 靶点：
MAG/Siglec-4a
- 抗体英文名：
Rat MAG/Siglec-4a Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Rat MAG/Siglec-4a Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
86073-5-PBS targets MAG/Siglec-4a as part of a matched antibody pair:
MP02605-1: 86073-5-PBS capture and 86073-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA, Sample test |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg3251 Product name: Recombinant Rat MAG protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 20-516 aa of NM_017190.4 Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | myelin-associated glycoprotein |
| 别名 | 1B236, Brain neuron cytoplasmic protein 3, Mag, Myelin-associated glycoprotein, Sialic acid-binding Ig-like lectin 4a |
| 计算分子量 | 69 kDa |
| GenBank蛋白编号 | NM_017190.4 |
| 基因名称 | Mag |
| Gene ID (NCBI) | 29409 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07722-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验, then permeabilize with 0.5% Triton X-100 in PBS, for 5min at RT. (Note: if necessary, lower detergent concentrations, e.g. 0.1-0.2%, may be used). 3. Remove permeabilization solution and wash samples twice with PBS, 3-5min each wash. During washes or blocking step
PBS-T洗板4次. 甩去液体. 用纸吸干； ④ 上二抗：加入Biotin-绵羊抗人I g g单克隆抗体（1:1000）. 37 ℃湿盒温育1 h. 1×PBS-T洗板4次. 甩去液体. 用纸吸干； ⑤ 上标记物：加入Avidin- hRP（1:1000）. 37 ℃湿盒温育30 Min. 1×PBS-T洗板4次. 甩去液体. 用纸吸干； ⑥ 显色：T MB避光显色15 Min. 加1N h2SO4终止反应； ⑦ 酶标仪OD450
第一章 细胞培养的基本原理与技术现代生物技术一般认为包括基因工程技术、细胞工程技术、酶工程技术和发酵工程技术，而这些技术的发展几乎都与细胞培养有密切关系，特别是在医药领域的发展，细胞培养更具有特殊的作用和价值。比如基因工程药物或疫苗在研究生产过程中很多是通过细胞培养来实现的。基因工程乙肝疫苗很多是以CHO细胞作为载体；细胞工程中更是离不细胞培养，杂交瘤单克隆抗体，完全是通过细胞培养来实现的，既使是现在飞速发展的基因工程抗体也离不开细胞培养。正在倍受重视的基因治疗、体细胞治疗也要经过细胞培养过程
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