- ￥5800
- Proteintech
- 67028-1-PBS
- 2026年05月29日
- WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RPL4 protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
- 靶点：
Ribosomal protein L4
- 抗体英文名：
Ribosomal protein L4 Monoclonal antibody, PBS Only
- 抗体名：
Ribosomal protein L4 Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
67028-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag28728 Product name: Recombinant human RPL4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-427 aa of BC009888 Sequence: MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAALQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKKAAATKKPAPEKKPAEKKPTTEEKKPAA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L4 |
| 别名 | RPL4, 3C7A8, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC009888 |
| 基因名称 | Ribosomal protein L4 |
| Gene ID (NCBI) | 6124 |
| RRID | AB_2882343 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P36578 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
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文献和实验少可以参考文献上给定的硫酸铵固体的溶解度表，《Guide to Protein Purification》一书中就有详细的溶解度表，静置两个小时左右，10000 g离心半个小时后，弃去上清，用PBS重新溶解沉淀，然后再次加入硫酸铵固体至45%饱和，静置两个小时左右10000 g离心半个小时，弃去上清，用少量PBS溶解沉淀，即得到较纯的抗体，因为此抗体中尚有较高浓度的硫酸铵，因此需要对PBS透析，一般透析一天一夜较合适，透析过程中更换透析液两至三次。如果对纯度要求不是很高，也可以不做第一次沉淀，直接用45
members, including fibrillarin and nucleolin, involved in ribosomal biogenesis, histones, fibroblast growth factor, interleukin enhancer-binding factor 3 the SRC kinase adapter protein Sam68, STAT1, and EWS. Analysis of protein arginine methylation
Protein G Purification of Antibodies
the protein concentration of the antibody. Concentrate if necessary. Store aliquots at -20o C. 上一篇：一种提高单克隆抗体产量的简易方法 下一篇：Protein A Purification of Antibody
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