- ￥5800
- Proteintech
- 60789-3-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SFTPA1 protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
Surfactant Protein A
- 抗体英文名：
Surfactant Protein A Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Surfactant Protein A Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
60789-3-PBS targets Surfactant Protein A as part of a matched antibody pair:
MP51129-2: 60789-1-PBS capture and 60789-3-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23599 Product name: Recombinant human SFTPA1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 132-248 aa of BC026229 Sequence: MTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | surfactant protein A1 |
| 别名 | SFTPA1, SFTPA2, 35 kDa pulmonary surfactant-associated protein, COLEC4, Collectin 4 |
| 计算分子量 | 248 aa, 26 kDa |
| GenBank蛋白编号 | BC026229 |
| 基因名称 | Surfactant Protein A |
| Gene ID (NCBI) | 653509 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8IWL2 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验少可以参考文献上给定的硫酸铵固体的溶解度表，《Guide to Protein Purification》一书中就有详细的溶解度表，静置两个小时左右，10000 g离心半个小时后，弃去上清，用PBS重新溶解沉淀，然后再次加入硫酸铵固体至45%饱和，静置两个小时左右10000 g离心半个小时，弃去上清，用少量PBS溶解沉淀，即得到较纯的抗体，因为此抗体中尚有较高浓度的硫酸铵，因此需要对PBS透析，一般透析一天一夜较合适，透析过程中更换透析液两至三次。如果对纯度要求不是很高，也可以不做第一次沉淀，直接用45
Protein G Purification of Antibodies
the protein concentration of the antibody. Concentrate if necessary. Store aliquots at -20o C. 上一篇：一种提高单克隆抗体产量的简易方法 下一篇：Protein A Purification of Antibody
-Buffer® protein-free as sample diluent, and HRP-Protector™ protein-free or LowCross® HRP-Stab protein-free as detector antibody diluents, enable the development and manufacturing of completely animal-free assays. The advantages of specialized
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