- ￥5800
- Proteintech
- 86658-1-PBS
- 2025年10月09日
- WB, IHC, Indirect ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CSH2 protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IHC, Indirect ELISA
- 靶点：
Placental lactogen
- 抗体英文名：
Placental lactogen Recombinant monoclonal antibody, PBS Only
- 抗体名：
Placental lactogen Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
86658-1-PBS targets Placental lactogen in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9412 Product name: Recombinant human CSH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC022044 Sequence: MAAGSRTSLLLAFALLCLPWLQEAGAVQTVPLSRLFDHAMLQAHRAHQLAIDTYQEFEETYIPKDQKYSFLHDSQTSFCFSDSIPTPSNMEETQQKSNLELLRISLLLIESWLEPVRFLRSMFANNLVYDTSDSDDYHLLKDLEEGIQTLMGRLEDGSRRTGQILKQTYSKFDTNSHNHDALLKNYGLLYCFRKDMDKVETFLRMVQCRSVEGSCGF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chorionic somatomammotropin hormone 2 |
| 别名 | CSH2, Choriomammotropin, CS 2, CSB, CSH1 |
| 计算分子量 | 217 aa, 25 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC022044 |
| 基因名称 | CSH2 |
| Gene ID (NCBI) | 1443 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01243 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus.
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文献和实验Staining protocols for placental explant cultures
objectives. Whole mount staining in multiwell plates. Method Wash the cultures gently and thoroughly in PBS twice. Fix in 1 ml MeOH. Replace the first solution immediately with a second aliquot. Incubate 30 min. Wash three times with PBS. At this stage
Human Placental Alkaline Phosphatase Stain
buffered saline (PBS) for 5-10 min. It is not necessary to wash the cells with PBS prior to fixation. 2. WASH: Wash cells 3 times with PBS. 3. HEAT: Heat cells at 65°C for 30 min in PBS to inactivate heat-labile AP in cells. Human placental AP
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
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