- ￥5800
- Proteintech
- 83728-1-PBS
- 2025年10月09日
- Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Apolipoprotein E
- 抗体英文名：
Mouse Apolipoprotein E Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Mouse Apolipoprotein E Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
83728-1-PBS targets Apolipoprotein E as part of a matched antibody pair:
MP00712-1: 83728-3-PBS capture and 83728-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1093 Product name: Recombinant Mouse APOE protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-311 aa of NM_001305819.1 Sequence: EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Apolipoprotein E |
| 别名 | Apoe, AI255918 |
| 计算分子量 | 36kDa |
| GenBank蛋白编号 | NM_001305819.1 |
| 基因名称 | Apoe |
| Gene ID (NCBI) | 11816 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08226 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验E L I S A w i t h p l a t e l e t s
prepare platelets from control and immunized mice distribute 2.5 x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. fix with PBS-PF
Pathology and Autopsy of a mouse
once (2-24h) with PBS and transfer to 70% ethanol for less than a week storage. Bigger specimens (e.g. whole mouse) need 24 to 48h fixation, one day in PBS and then two changes (6-12 hrs apart) of 70% ethanol for short and long term storage.
过夜。（5）用少许PBS将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟；再加入3/20V NaBH4溶液，混匀，室温作用1小时（或4℃过夜）。（6）将交联物过Sephadex G200或Sepharose 6B（2.6×50cm）层析纯化，分管收集第一峰。（7）酶结合物质量鉴定：克分子比值测定酶量（mg/ml）=OD403×0.4IgG量（mg/ml）=（OD280-OD403×0.3）×0.62克分子比值（E/P）=酶量×4/IgG量
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