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KDM5C 重组单克隆抗体, PBS Only (Captu

re) 86017-3-PBS
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  • ¥5800
  • Proteintech已认证
  • 86017-3-PBS
  • 2025年10月09日
  • Sandwich ELISA, Indirect ELISA, Sample test
  • Rabbit
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant human KDM5C protein

    • 亚型

      IgG

    • 保存条件

      Store at -80°C.

    • 克隆性

      Recombinant

    • 标记物

      Unconjugated

    • 适应物种

      human

    • 宿主

      Rabbit

    • 应用范围

      Sandwich ELISA, Indirect ELISA, Sample test

    • 靶点

      KDM5C

    • 抗体英文名

      KDM5C Recombinant monoclonal antibody, PBS Only (Capture)

    • 抗体名

      KDM5C Recombinant monoclonal antibody, PBS Only (Capture)

    • 规格

      100ug

    产品信息

    86017-3-PBS targets KDM5C as part of a matched antibody pair:

    MP02624-1: 86017-3-PBS capture and 86017-2-PBS detection (validated in Sandwich ELISA)

    Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

    This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

    经测试应用 Sandwich ELISA, Indirect ELISA, Sample test
    经测试反应性 human
    免疫原

    CatNo: Ag5791

    Product name: Recombinant human KDM5C protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 1-351 aa of BC054499

    Sequence: MEPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEVDNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERRILDLYSLSKIVVEEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPYEMYQSGANLVCNTRPFDNEEKDKEYKPHSIPLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYGAGPKMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLESKEELSHSPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLLCDGCDDNYHIF

    宿主/亚型 Rabbit / IgG
    抗体类别 Recombinant
    产品类型 Antibody
    全称 lysine (K)-specific demethylase 5C
    别名 [histone H3]-trimethyl-L-lysine(4) demethylase 5C, DXS1272E, EC:1.14.11.67, JARID1C, Jumonji/ARID domain-containing protein 1C
    计算分子量 176 kDa
    GenBank蛋白编号BC054499
    基因名称 KDM5C
    Gene ID (NCBI) 8242
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Protein A purification
    UNIPROT IDP41229
    储存缓冲液 PBS only, pH 7.3.
    储存条件Store at -80°C.
    The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

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    图标文献和实验
    相关实验
    • PBS缓冲液等常用缓冲溶液的配制方法

      2 O分子量 = 178.05,0.2 mol/L溶液含35.01克/升。 C 4 H 2 O 7 ·H 2 O分子量 = 210.14,0.1 mol/L溶液为21.01克/升。 4. 柠檬酸–氢氧化钠-盐酸缓冲液 使用时可以每升中加入1克克酚,若最后pH值有变化,再用少量50% 氢氧

    • Chromosome Conformation Capture (3C) (alternate technique)

      Wash with 50ml cold PBS and centrifuge at 1300 rpm for 8 minutes at 4°C; lysis buffer and incubate on ice for 30 minutes with occasional mixing; Centrifuge at 1800 rpm for 5 minutes at 4°C and resuspend nuclei in trace of liquid;

    • Actin Capture Assay

      . For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA

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