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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
IL-12/IL-23 p40
- 抗体英文名:
Mouse IL-12/IL-23 p40 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名:
Mouse IL-12/IL-23 p40 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格:
100ug
产品信息
82865-9-PBS targets IL-12/IL-23 p40 as part of a matched antibody pair:
MP00413-2: 82865-9-PBS capture and 82865-7-PBS detection (validated in Cytometric bead array)
MP00413-3: 82865-9-PBS capture and 82865-6-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0299 Product name: Recombinant mouse Il12b protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 23-335 aa of NM_001303244 Sequence: MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 12b |
| 别名 | Il12b, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Il 12b, Il 12p40, IL-12 p40 |
| 计算分子量 | 38kd |
| GenBank蛋白编号 | NM_001303244 |
| 基因名称 | Il12b |
| Gene ID (NCBI) | 16160 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43432 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
情况。PeproTech的大多数细胞因子或蛋白冻干粉的溶解液不是PBS,此时千万不能用PBS或培养液直接来溶解,具体原因在上面已经叙述过。而有部分细胞因子,如重组人KGF(产品编号:100-19)和重组人FGF-23(产品编号:100-52)等,说明书上的溶解液即为1x PBS,此时用PBS溶解完全没问题,那么用培养液溶解也是可以的,不过最好还是用先用PBS溶解,然后再用培养液稀释。 2) 一定要溶解到指定的浓度。 蛋白在一定的浓度范围内可以保持良好的稳定性,这个范围就是说明书上所标明的浓度
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