- ￥920 - 2310
- Proteintech
- CR-65086
- 2025年10月09日
- FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
Human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD11c
- 抗体英文名：
Cardinal Red™ Anti-Human CD11c (3.9)
- 抗体名：
Cardinal Red™ Anti-Human CD11c (3.9)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥920.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2310.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65086 targets CD11c in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin, alpha X (complement component 3 receptor 4 subunit) |
| 别名 | CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6 |
| 计算分子量 | 1169 aa, 129 kDa |
| GenBank蛋白编号 | BC038237 |
| 基因名称 | CD11c |
| Gene ID (NCBI) | 3687 |
| ENSEMBL Gene ID | ENSG00000140678 |
| RRID | AB_2935223 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P20702 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).
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文献和实验Intracellular Cytokine Staining Protocol
are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required for detection of cytokines
Intracellular Cytokine Staining Protocol
. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required
.67144-12.7394-17.1Human1724.1846.7-4.183.785.1633.5-7.5168-2.2Human229.8-2.6829.8-2.7625.1-5.08151-11.7287-9.1Bio-Red1630391.287.1024-14.62522Bio-Red2421.7927018.0-4.44201-14.32980.84* 从总蛋白结果来看RANBOX与Bio-Red 相差为6.45%，而宝灵曼与Bio-Red 较为接近；* 从白蛋白结果分析，宝灵曼与Bio
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