- ￥5800
- Proteintech
- 66832-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NRF1 protein
- 亚型：
IgG2b
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
- 靶点：
NRF1/nuclear respiratory factor 1
- 抗体英文名：
NRF1/nuclear respiratory factor 1 Monoclonal antibody, PBS Only
- 抗体名：
NRF1/nuclear respiratory factor 1 Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
66832-1-PBS targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag28149 Product name: Recombinant human NRF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 213-522 aa of BC016925 Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nuclear respiratory factor 1 |
| 别名 | NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein |
| 计算分子量 | 522 aa, 56 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC016925 |
| 基因名称 | NRF1 |
| Gene ID (NCBI) | 4899 |
| RRID | AB_2882175 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16656 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
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文献和实验的产量且方法简便［3］。经我们对3株抗人衰变加速因子（Decay-Accelerating Factor，DAF，CD55）单克隆抗体的制备和产量测定，获得较为满意的结果，现报道如下。 1 材料与方法 1.1 纯化抗原 及抗体 纯化的可溶性重组DAF由英国Reading大学Goodfellow博士惠赠。抗人DAF单克隆抗体IC6培养上清（1μg／ml）由日本Fukushima医学院Fujita博士惠赠。辣根过氧化物酶标记的羊抗鼠IgG（工作浓度1∶1000），购自
-Accelerating Factor，DAF，CD55）单克隆抗体的制备和产量测定，获得较为满意的结果，现报道如下。一、材料与方法1、纯化抗原及抗体纯化的可溶性重组DAF由英国Reading大学Goodfellow博士惠赠。抗人DAF单克隆抗体IC6培养上清（1μg／ml）由日本Fukushima医学院Fujita博士惠赠。辣根过氧化物酶标记的羊抗鼠IgG（工作浓度1∶1000），购自中山生物制品有限公司。2、培养基RPMI 1640（Gibco产品），含2mmol L-谷氨酰胺，100U／ml青霉素，100μg／
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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