- ￥5800
- Proteintech
- 65277-1-PBS
- 2025年10月09日
- FC (Intra)
- Mouse
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
-20°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC (Intra)
- 靶点：
IL-12A/IL-12 p35
- 抗体英文名：
Anti-Human IL-12A/IL-12 p35 (1E12H6), PBS Only
- 抗体名：
Anti-Human IL-12A/IL-12 p35 (1E12H6), PBS Only
- 规格：
100ug
产品信息
65277-1-PBS targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human |
| 免疫原 | fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) |
| 别名 | IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35 |
| 计算分子量 | 25 kDa |
| GenBank蛋白编号 | BC104982 |
| 基因名称 | IL-12A |
| Gene ID (NCBI) | 3592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29459 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | -20°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
技术资料暂无技术资料 索取技术资料
