- ￥1500 - 3280
- Proteintech
- 82902-1-RR
- 2025年10月09日
- WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
- 靶点：
Acetyl-Histone H3 (Lys27)
- 抗体英文名：
Acetyl-Histone H3 (Lys27) Recombinant monoclonal antibody
- 抗体名：
Acetyl-Histone H3 (Lys27) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells, mouse kidney tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:19600 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82902-1-RR targets Acetyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Ac Histone H3, Ac Histone H3 (Lys27), Ac Histone H3 Lys27, Ac Histone H3-Lys27, Acetyl Histone |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3670631 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.
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文献和实验| Species | Application | Title |
|---|---|---|
| rat,mouse | WB | J Med Chem Discovery of Novel Imidazo[1,2-a]pyridine-Based HDAC6 Inhibitors as an Anticarcinogen with a Cardioprotective EffectAuthors - Fei-Fei Yang |
| human | WB | Cell Death Discov The dual HDAC/PI3K inhibitor CUDC-907 inhibits the growth and proliferation of MYC-driven Group 3 medulloblastomaAuthors - Pan Gou |
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
Identifying Differential Histone Modification Sites from ChIPseq Data
a hidden Markov model (HMM) to infer the states of histone modification changes at each genomic location. We evaluated the performance of ChIPDiff by comparing the H3K27me3 modification sites between mouse embryonic stem cell (ESC) and neural progenitor
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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