- ￥930 - 2320
- Proteintech
- CL750-65074
- 2025年10月09日
- FC (Intra)
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC (Intra)
- 靶点：
IL-4
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse IL-4 (11B11)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse IL-4 (11B11)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥930.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2320.0 |
经过测试的应用
| Positive FC (Intra) detected in | Anti-CD3/CD28-stimulated mouse splenocytes |
| Positive FC detected in | Anti-CD3/CD28-stimulated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 4 |
| 别名 | Il 4, Il4, interleukin 4 |
| GenBank蛋白编号 | BC027514 |
| 基因名称 | IL-4 |
| Gene ID (NCBI) | 16189 |
| RRID | AB_3084840 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P07750 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
Brefeldin A BVD6-24G2 , 11B11 IL-5 NEW! mouse splenic CD4 ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hr
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
This protocol is based upon protocols from Mark Biggin, Dave Allis and Richard Treisman plus a fair amount of trial and error. We have successfully used this protocol with livers, spleens, colons and whole mouse embryos
二、超声波处理法 1、要设定好超声时间和间隙时间，一般超声时间不超过5秒，间隙时间最好大于超声时间，这些都有利于保护酶的活性。我的实验超声时间5秒、间隙时间10秒、工作次数20次。 2、每个样本超声时间5秒,每个间隔5秒,粉碎5次。 3、100ml菌液离心，用8ml缓冲液悬浮，加8mg溶菌酶，冰浴30min，然后超声处理。750W 40％功率。5秒超声，9秒间隔。超声至少90min。 4、综合冻融和超声的方法： 1500g离心，弃除上清。冰上，用小体
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