- ￥860 - 2160
- Proteintech
- CR-65132
- 2025年10月09日
- FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
Human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD27
- 抗体英文名：
Cardinal Red™ Anti-Human CD27 (O323)
- 抗体名：
Cardinal Red™ Anti-Human CD27 (O323)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥860.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2160.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65132 targets CD27 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD27 molecule |
| 别名 | CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55 |
| 计算分子量 | 29 kDa |
| GenBank蛋白编号 | BC012160 |
| 基因名称 | CD27 |
| Gene ID (NCBI) | 939 |
| ENSEMBL Gene ID | ENSG00000139193 |
| RRID | AB_3084955 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P26842 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.
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文献和实验in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0% BSA/PBS, 2.0% normal horse serum. Prepare
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
banks of hESC lines with matched or reduced incompatibility could potentially reduce or eliminate the need for immunosuppressive drugs and/or immunomodulatory protocols altogether, for example, O-type RhD− lines for generation of universal red blood
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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