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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
Sandwich ELISA, Indirect ELISA, Sample test
- 靶点:
CD107b / LAMP2
- 抗体英文名:
Mouse CD107b / LAMP2 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Mouse CD107b / LAMP2 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
84474-4-PBS targets CD107b / LAMP2 as part of a matched antibody pair:
MP01356-4: 84474-2-PBS capture and 84474-4-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2029 Product name: Recombinant Mouse CD107b/LAMP2 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-379 aa of NM_001017959.2 Sequence: LIVNLTDSKGTCLYAEWEMNFTITYETTNQTNKTITIAVPDKATHDGSSCGDDRNSAKIMIQFGFAVSWAVNFTKEASHYSIHDIVLSYNTSDSTVFPGAVAKGVHTVKNPENFKVPLDVIFKCNSVLTYNLTPVVQKYWGIHLQAFVQNGTVSKNEQVCEEDQTPTTVAPIIHTTAPSTTTTLTPTSTPTPTPTPTPTVGNYSIRNGNTTCLLATMGLQLNITEEKVPFIFNINPATTNFTGSCQPQSAQLRLNNSQIKYLDFIFAVKNEKRFYLKEVNVYMYLANGSAFNISNKNLSFWDAPLGSSYMCNKEQVLSVSRAFQINTFNLKVQPFNVTKGQYSTAQDCSADEDN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lysosomal-associated membrane protein 2 |
| 别名 | CD107b, Lamp2, Lamp 2, Mac3 |
| 计算分子量 | 46 kDa |
| GenBank蛋白编号 | NM_001017959.2 |
| 基因名称 | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17047-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
Isolation of Primary Fibroblasts from Mouse Embryos
1.Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2.Sacrifice the pregnant female mouse (day 13 or 14 p.c.) by cervical dislocation. Dissect out the uterine horns and place into a petri dish containing PBS
Isolation of Primary Fibroblasts from Mouse Embryos
and proliferate.) 9. At confluence, split the plate 1:3 by doing the following: a. Wash the plate twice with PBS. b. Add 2 ml of trypsin per 10 cm dish and incubate @ 37o C for 7 minutes. c. Add 5 ml of medium (DMEM, 1X GPS, and 10
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