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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
rat
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD45RA
- 抗体英文名:
Anti-Rat CD45RA (OX-33)
- 抗体名:
Anti-Rat CD45RA (OX-33)
- 规格:
100ug/500ug
| 规格: | 100ug | 产品价格: | ¥1070.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥3350.0 |
经过测试的应用
| Positive FC detected in | Wistar rat splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65234-1-Ig targets CD45RA in FC applications and shows reactivity with rat samples.
| 经测试应用 | FC |
| 经测试反应性 | rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | CD45, Lca, Ptprc, L CA, OX-33 |
| 计算分子量 | 143 kDa |
| GenBank蛋白编号 | XM_006249910 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 24699 |
| RRID | AB_2935147 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P04157 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
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膜上有关;后者与抗原信号传递和Ca2+流动有关。其中的极性氨基酸(Thr、Ser)与抗原识别相关分子之间形成盐键,以稳定BCR复合结构。mlg的胞内区极短,mlgM和mlgD均只有3个氨基酸残基,与T细胞中的TCR相类似,BCR需要与Iga(CD79a)和Igp(CD79b)以非共价结合形式构成复合体,共同完成对抗原的识别及信号转导。 B细胞和T细胞抗原识别结构的主要成分比较 Igα和Igβ为单链I型跨膜蛋白,分子质量分别是33kDa和37kDa,以二硫
(Ig E ) IL-5 辅助型T细胞 (TH2-cells) 角色分化的B细胞,嗜酸性粒细胞(eosinophil)生成,和 Ig A 生成 IL-6 巨噬细胞(macrophages), 辅助型T细胞(TH 2-cells ) 引起急性期反应 IL-7
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