- ￥770 - 1930
- Proteintech
- CR-65235
- 2025年10月09日
- FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
Human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD71
- 抗体英文名：
Cardinal Red™ Anti-Human CD71 (OKT9)
- 抗体名：
Cardinal Red™ Anti-Human CD71 (OKT9)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥770.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥1930.0 |
经过测试的应用
| Positive FC detected in | PHA treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65235 targets CD71 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transferrin receptor (p90, CD71) |
| 别名 | CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR |
| 计算分子量 | 85 kDa |
| GenBank蛋白编号 | BC001188 |
| 基因名称 | CD71 |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_3084984 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P02786 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
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文献和实验T-Cell Activation Using mAb to CD3
for stimulation of PBMC. Materials 1X sterile PBS Anti-human CD3: Clone OKT3 (Functional Grade Cat. No. 16-0037 ) or Clone HIT3a (Functional Grade Cat. No. 16-0039 ) Complete RPMI-1640 Sterile PBMC 96
3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. What You Need Materials 1X sterile PBS Anti-human CD3: OKT3, Functional Grade Cat
Intracellular Cytokine Staining Protocol
are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required for detection of cytokines
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