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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, FC (Intra), Indirect ELISA
- 靶点:
Cathepsin B
- 抗体英文名:
Mouse Cathepsin B Recombinant monoclonal antibody, PBS Only
- 抗体名:
Mouse Cathepsin B Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
85841-5-PBS targets Cathepsin B in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg3028 Product name: Recombinant Mouse Ctsb protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 18-339 aa of NM_007798.3 Sequence: HDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cathepsin B |
| 别名 | Cathepsin B heavy chain, Cathepsin B light chain, Cathepsin B1, Ctsb, EC:3.4.22.1 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 31 kDa, 39 kDa |
| GenBank蛋白编号 | NM_007798.3 |
| 基因名称 | Ctsb |
| Gene ID (NCBI) | 13030 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10605 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 39 kDa form of pro-CTSB, 31 kDa mature forms in mouse brain by western blot.
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文献和实验相关。 可以选择的羧肽酶B 有两种,一种是动物胰腺提取的羧肽酶B,一种是基因工程的重组羧肽酶B,前者由于胰腺中含有各种蛋白酶,如胰蛋白酶、糜蛋白酶等,常常不能彻底去除,在酶切时会有非特异的酶切杂峰出现,影响结果判断。后者为基因工程生产的,成分单一,无杂酶,比活高,应用上更有优势。 羧肽酶 B 的结构 天然存在的羧肽酶B 是由前羧肽酶原B(preprocarboxypeptidase B,preproCPB)经酶解产生,后者 N-末端包含 108 个氨基酸(其中 13 个氨基酸为信号
the Swiss Webster or CD1 outbred strains, although past usage includes both C57Bl/6 and B6C3F1. The Anesthetic Avertin (2,2,2 tribromoethanol) is found to be quite effective. For method of preparation, see "Manipulating the Mouse Embryo", CSHL
上带有Ampr 和lacZ基因,故重组子的筛选采用Amp抗性筛选与α-互补现象筛选相结合的方法。 因pBS带有Ampr 基因而外源片段上不带该基因,故转化受体菌后只有带有pBS DNA的转化子才能在含有Amp的LB平板上存活下来;而只带有自身环化的外源片段的转化子则不能存活。此为初步的抗性筛选。 pBS上带有β-半乳糖苷酶基因(lacZ)的调控序列和β-半乳糖苷酶N端146个氨基酸的编码序列。这个编码区中插入了一个多克隆位点,但并没有破坏
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