- ￥1200
- Proteintech
- CL750-84814
- 2025年10月09日
- IF/ICC
- Rabbit
- recombinant protein
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- 详细信息
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
recombinant protein
- 宿主：
Rabbit
- 应用范围：
IF/ICC
- 靶点：
6*his,His tag
- 抗体英文名：
CoraLite® Plus 750-conjugated 6*his,His tag Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 750-conjugated 6*his,His tag Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-84814 targets 6*his,His tag in IF/ICC applications and shows reactivity with recombinant protein samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | recombinant protein |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | 6*his,His tag |
| 别名 | 6*His, His-Tag, 6*His, His Tag, His Tag, 241261A7, 6*His |
| 计算分子量 | 1 kDa |
| 基因名称 | |
| Gene ID (NCBI) | 5 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | HISTAG |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
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