- ￥2800
- Proteintech
- CL488-80545
- 2025年10月09日
- IF/ICC
- Rabbit
- Human, Mouse, Pig
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human Occludin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
Human, Mouse, Pig
- 宿主：
Rabbit
- 应用范围：
IF/ICC
- 靶点：
Occludin
- 抗体英文名：
CoraLite® Plus 488-conjugated Occludin Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Occludin Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80545 targets Occludin in IF/ICC applications and shows reactivity with Human, Mouse, Pig samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | Human, Mouse, Pig |
| 免疫原 |
CatNo: Ag26173 Product name: Recombinant human Occludin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-522 aa of BC029886 Sequence: VDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | occludin |
| 别名 | occludin, OCLN |
| 计算分子量 | 522 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC029886 |
| 基因名称 | Occludin |
| Gene ID (NCBI) | 4950 |
| RRID | AB_3084489 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16625 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
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