- ￥5800
- Proteintech
- 82832-1-PBS
- 2025年10月09日
- WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR
- Rabbit
- human, mouse, rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR
- 靶点：
Acetyl-Histone H3 (Lys18)
- 抗体英文名：
Acetyl-Histone H3 (Lys18) Recombinant monoclonal antibody, PBS Only
- 抗体名：
Acetyl-Histone H3 (Lys18) Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
82832-1-PBS targets Acetyl-Histone H3 (Lys18) in WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Ac Histone H3, Ac Histone H3 (Lys18), Ac Histone H3 Lys18, Ac Histone H3-Lys18, Acetyl Histone |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Histone Assay Solution (10 rxns) Amount [Final] Histone Wash Buffer 320 µL - Histone H1, 2 µg/µL 18 µl 0.1 µg/µL
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP,可观察到绿色荧光）。四、重组病毒鉴定 1、转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清，感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d，当1/3-1/2细胞漂浮时
技术资料暂无技术资料 索取技术资料
